Human Integrin beta1/CD29 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17781
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Papio cynocephalus (Yellow Baboon)
Applications
Validated:
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Knockout Validated
Cited:
Bioassay, Blocking, Flow Cytometery, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P5D2
Product Specifications
Immunogen
Human skin keratinocytes
Specificity
Detects human Integrin beta1/CD29.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Integrin beta1/CD29 Antibody
Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Detection of Integrin beta1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.
A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.Integrin beta1/CD29 in human PBMCs.
Integrin beta1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin beta1/CD29 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450.
Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and A549 human lung carcinoma cell line
Sample: Human peripheral blood mononuclear cells (PBMCs) and A549 human lung carcinoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Immunoprecipitation
Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
Joneckis, C.C. et al. (1993) Blood 82:3548.
Knockout Validated
Integrin beta1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta1/CD29 knockout A549 cell line.
Reviewed Applications
Read 5 reviews rated 4.6 using MAB17781 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
References
- Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
- Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
Additional Integrin beta 1/CD29 Products
Product Documents for Human Integrin beta1/CD29 Antibody
Product Specific Notices for Human Integrin beta1/CD29 Antibody
For research use only
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