Human Integrin  beta1/CD29 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB17781P

R&D Systems, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Hamster - Cricetulus (Chinese Hamster), Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Flow Cytometry, Knockout Validated

Cited:

Flow Cytometry, Neutralization

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P5D2

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Human skin keratinocytes

Specificity

Detects human Integrin beta1/CD29 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  beta1/CD29 PE-conjugated Antibody

Detection of Integrin beta1/CD29 antibody in A549 human lung carcinoma cell line antibody by Flow Cytometry.

Detection of Integrin beta1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin beta1/CD29 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Integrin  beta1/CD29 Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

Integrin  beta1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Integrin beta1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the Integrin beta1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  beta1/CD29 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Knockout Validated

Integrin beta1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta1/CD29 knockout A549 cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 1/CD29

The Integrin beta1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

References

  1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
  2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Human Integrin  beta1/CD29 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  beta1/CD29 PE-conjugated Antibody

For research use only

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