Human Integrin beta2/CD18 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1730
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Adhesion Blockade, CyTOF-ready, Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Migration Assay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta2
Gln23-Asn700
Accession # AAA59490
Gln23-Asn700
Accession # AAA59490
Specificity
Detects human Integrin beta2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Integrin beta2/CD18 Antibody
Integrin beta2/CD18 in Human PBMCs.
Integrin beta2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Integrin beta2/CD18 in THP-1 cells (Positive) & absent in RT-4 cells (Negative).
Integrin beta2/CD18 was detected in immersion fixed THP-1 human acute monocytic leukemia cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma (Negative) using Goat Anti-Human Integrin beta2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Integrin beta2/CD18 Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells
Sample: Human peripheral blood mononuclear cells
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs), THP-1 human acute monocytic leukemia cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma cells (Negative)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs), THP-1 human acute monocytic leukemia cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma cells (Negative)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 2/CD18
Integrin beta2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alphaD, alphaL (complex also known as LFA‑1), alphaM (Mac-1), or alphaX (CR-4). These complexes are important in adhesion and leukocyte trafficking.
Alternate Names
CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta
Gene Symbol
ITGB2
UniProt
Additional Integrin beta 2/CD18 Products
Product Documents for Human Integrin beta2/CD18 Antibody
Product Specific Notices for Human Integrin beta2/CD18 Antibody
For research use only
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