Human Integrin beta6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4155
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 437211
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alphaV beta6
Phe31-Val992 (Integrin alphaV) and Gly22-Asn707 (Integrin beta6)
Accession # P18564
Phe31-Val992 (Integrin alphaV) and Gly22-Asn707 (Integrin beta6)
Accession # P18564
Specificity
Detects human Integrin beta6 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Integrin beta6 Antibody
Detection of Integrin beta6 in HT-29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Human Integrin beta6 Monoclonal Antibody (Catalog # MAB4155, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Integrin beta6 in HT‑29 Human Cell Line.
Integrin beta6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Integrin beta6 Monoclonal Antibody (Catalog # MAB4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Integrin beta6 in HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells .
Integrin beta6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive) and A549 human lung carcinoma cell line (negative) cells using Mouse Anti-Human Integrin beta6 Monoclonal Antibody (Catalog # MAB4155) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Integrin beta6 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells
Sample: Immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 6
Integrin beta6 (ITG beta6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alphaV and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta6 ECD, respectively.
Alternate Names
ITGB6
Gene Symbol
ITGB6
UniProt
Additional Integrin beta 6 Products
Product Documents for Human Integrin beta6 Antibody
Product Specific Notices for Human Integrin beta6 Antibody
For research use only
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