Human IRF8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5117
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IRF8
Ala255-Val426
Accession # Q02556
Ala255-Val426
Accession # Q02556
Specificity
Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human IRF1, 2, 3, 4, 5, 6, 7, and 9.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human IRF8 Antibody
Detection of Human IRF8 by Western Blot.
Western blot shows lysates of human liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
IRF8 in Human Lymphoma.
IRF8 was detected in immersion fixed paraffin-embedded sections of human lymphoma using 5 µg/mL Sheep Anti-Human IRF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5117) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human IRF8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Western Blot
1 µg/mL
Sample: Human liver and kidney tissue
Sample: Human liver and kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF8
IRF8 (interferon regulatory factor 8; also ICSBP) is a 50 kDa member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL-18, IL-12p35, and PTPN13, a Fas-associated phosphatase that blocks Fas-induced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 9-110) and at least one regulatory phosphorylation site at Tyr95. Over aa 255-426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively.
Long Name
Interferon Regulatory Factor 8
Alternate Names
ICSBP1
Gene Symbol
IRF8
UniProt
Additional IRF8 Products
Product Documents for Human IRF8 Antibody
Product Specific Notices for Human IRF8 Antibody
For research use only
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