Human Isocitrate Dehydrogenase 2/IDH2 Antibody

Detection of Isocitrate Dehydrogenase 2/IDH2 in Jurkat cells by Flow Cytometry

Jurkat cells were stained with Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Isocitrate Dehydrogenase 2/IDH2 in MCF-7 cells (Positive) and SK-MEL-28 cells (Positive).

Isocitrate Dehydrogenase 2/IDH2 was detected in fixed MCF-7 human breast cancer cell line (Positive) and SK-Mel-28 human malignant melanoma cell line (Positive) using Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Isocitrate Dehydrogenase 2/IDH2 in Human Kidney.

Isocitrate Dehydrogenase 2/IDH2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and kidney tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.