Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Jak3 Monoclonal Antibody (Catalog # MAB46991, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Jak3 in Jurkat Human Cell Line.

Jak3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human Jak3 Monoclonal Antibody (Catalog # MAB46991) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Jak3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jak3

Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID).

Alternate Names

JAKL, LJAK

Entrez Gene IDs

3718 (Human); 16453 (Mouse); 25326 (Rat)

Gene Symbol

JAK3

UniProt

P52333

Additional Jak3 Products

Product Documents for Human Jak3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Jak3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Jak3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB46991

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Jak3 Antibodies »

Product Summary for Human Jak3 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Jak3 Antibody

Detection of Jak3 in Jurkat Human Cell Line by Flow Cytometry.