Human Kallikrein 3/PSA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13443

R&D Systems, part of Bio-Techne
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MAB13443-100
MAB13443-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 989729

View all Kallikrein 3/PSA Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Pro19-Pro261
Accession # P07288

Specificity

Detects human Kallikrein 3/PSA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Kallikrein 3/PSA Antibody

Detection of Human Kallikrein 3/PSA antibody by Western Blot.

Detection of Human Kallikrein 3/PSA by Western Blot.

Western blot shows lysates of human prostate tissue, human prostate cancer tissue, and DU145 human prostate carcinoma cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13443) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 3/PSA antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

Kallikrein 3/PSA in LNCaP Human Cell Line.

Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13443) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Kallikrein 3/PSA antibody in Human Prostate by Immunohistochemistry (IHC-P).

Kallikrein 3/PSA in Human Prostate.

Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13443) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Kallikrein 3/PSA Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample:

Immersion fixed LNCaP human prostate cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

2 µg/mL
Sample: Human prostate tissue and Human prostate cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB13443 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 3/PSA

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5-2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer. The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). Recombinant human PSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha1-antichymotrypsin (ACT) and alpha2-macroglobulin are known to form complexes with PSA.

References

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
  3. Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
  4. Lilja H. (2003) Urology 62:270.
  5. Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.

Alternate Names

KLK3, PSA

Entrez Gene IDs

354 (Human)

Gene Symbol

KLK3

UniProt

P07288

Additional Kallikrein 3/PSA Products

Product Documents for Human Kallikrein 3/PSA Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 3/PSA Antibody

For research use only

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