Human Ki67/MKI67 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7617
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Knockout Validated, Multiplex Immunofluorescence, Simple Western
Cited:
Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1297A
Product Specifications
Immunogen
Human/Mouse Ki67/MKI67 synthetic peptide
Accession # E9PVX6
Accession # E9PVX6
Specificity
Detects human Ki67/MKI67 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Ki67/MKI67 Antibody
Detection of Ki67/MKI67 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™
Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 10 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
Detection of Ki67 in Mouse Stomach via seqIF™ staining on COMET™
Ki67 was detected in immersion fixed paraffin-embedded sections of mouse Stomach using Rabbit Anti-Mouse Ki67, Monoclonal Antibody (Catalog # MAB7617) at 0.15ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
Ki67/MKI67 in HeLa Human Cell Line.
Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human Ki67/MKI67 Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-25 µg/mL
Sample: Formalin-fixed paraffin-embedded tissue sections of human breast cancer
Sample: Formalin-fixed paraffin-embedded tissue sections of human breast cancer
Immunocytochemistry
0.3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue and mouse thymus
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue and mouse thymus
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Knockout Validated
Ki67/MKI67 is specifically detected in Hela human cervical epithelial carcinoma parental cell line but is not detectable in Ki67/MKI67 knockout HeLa cell line.
Multiplex Immunofluorescence
0.15 - 10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil and mouse intestine
Sample: Immersion fixed paraffin-embedded sections of human tonsil and mouse intestine
Simple Western
20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line
Reviewed Applications
Read 2 reviews rated 4.5 using MAB7617 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ki67/MKI67
MKI67 (also Ki67) is a 350-400 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120 aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345 kDa in size and shows a deletion of aa 136-495, while a second isoform contains a 58 aa substitution for aa 1-513. Over aa 3120-3256, human Ki67 shares 46% aa sequence identity with the mouse ortholog to Ki67.
Long Name
Antigen Identified by Monoclonal Antibody Ki67
Alternate Names
Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126
Gene Symbol
MKI67
UniProt
Additional Ki67/MKI67 Products
Product Documents for Human Ki67/MKI67 Antibody
Product Specific Notices for Human Ki67/MKI67 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...