Human KLF10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7016
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human KLF10
Asn31-Ser154
Accession # Q13118
Asn31-Ser154
Accession # Q13118
Specificity
Detects human KLF10 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human KLF12 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human KLF10 Antibody
Detection of Human KLF10 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and IMR-32 human neuroblastoma cell line and cytoplasmic extracts of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF10 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human KLF10 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and IMR‑32 human neuroblastoma cell line
Sample: K562 human chronic myelogenous leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and IMR‑32 human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLF10
KLF10 (Kruppel-like factor 10; also TIEG1 and EGR-alpha) is a 55-65 kDa member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-beta, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-beta signaling. Human KLF10 is 480 amino acids (aa) in length and contains three C2H2-type zinc-finger motifs (aa 369-451). There is one potential alternate splice form that shows a deletion of aa 2-11. Over aa 31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF.
Long Name
Kruppel-Like Factor 10
Alternate Names
EGR-alpha, TIEG1
Gene Symbol
KLF10
UniProt
Additional KLF10 Products
Product Documents for Human KLF10 Antibody
Product Specific Notices for Human KLF10 Antibody
For research use only
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