Human KLF2 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC5466A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 665333
Product Specifications
Immunogen
E. coli-derived recombinant human KLF2
Pro71-Pro168 (predicted)
Accession # Q9Y5W3
Pro71-Pro168 (predicted)
Accession # Q9Y5W3
Specificity
Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross‑reactivity with rhKLF1, 4, 5, 6, 10, 12, recombinant mouse KLF4 or 15 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human KLF2 APC-conjugated Antibody
Detection of KLF2 in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 APC-conjugated Monoclonal Antibody (Catalog # IC5466A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human KLF2 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KLF2
KLF2 (Kruppel-Like Factor 2), also known as LKLF, is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family. KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells. It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length. It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354). There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.
Long Name
Kruppel-Like Factor 2
Alternate Names
LKLF
Gene Symbol
KLF2
UniProt
Additional KLF2 Products
Product Documents for Human KLF2 APC-conjugated Antibody
Product Specific Notices for Human KLF2 APC-conjugated Antibody
For research use only
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