Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross‑reactivity with rhKLF1, 4, 5, 6, 10, 12, recombinant mouse KLF4 or 15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human KLF2 PE-conjugated Antibody

Detection of KLF2 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 PE-conjugated Monoclonal Antibody (Catalog # IC5466P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human KLF2 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLF2

KLF2 (Kruppel-Like Factor 2), also known as LKLF, is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family. KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells. It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length. It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354). There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.

Long Name

Kruppel-Like Factor 2

Alternate Names

LKLF

Entrez Gene IDs

10365 (Human); 16598 (Mouse); 306330 (Rat)

Gene Symbol

KLF2

UniProt

Q9Y5W3 (Human)

Additional KLF2 Products

Product Documents for Human KLF2 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KLF2 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human KLF2 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC5466P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications