Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Lactate Dehydrogenase B/LDHB by Simple Western^TM.

Simple Western shows lysates of Exosome Standard (LNCaP) (NBP3-11687) and PC-3 human prostate cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 38 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Lactate Dehydrogenase B/LDHB in Human Liver.

Lactate Dehydrogenase B/LDHB was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line.

Lactate Dehydrogenase B/LDHB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Western Blot Shows Human Lactate Dehydrogenase B/LDHB Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LDHB knockout HEK293T cell line (KO). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Applications for Human Lactate Dehydrogenase B/LDHB Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

Lactate Dehydrogenase B/LDHB is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Lactate Dehydrogenase B/LDHB knockout HEK293Tcell line.

Simple Western

10 µg/mL
Sample: Exosome Standard (LnCAP) (Catalog # NBP3-11687) and PC-3 human prostate cancer cell line

Western Blot

0.05 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB9205 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitue at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lactate Dehydrogenase B/LDHB

LDHB is one of two subunits of Lactose Dehydrogenase which is formed from tetramer arrangements of the A and B subunits. LDHB is part of the pyruvate fermentation to lactate pathway in which it catalyzes the conversion of L-lactate and NAD to pyruvate and NADH. LDHA is widely expressed, with high expression in muscle and brain. High expression in tumor cells correlates with low survival rates in various cancers including osteosarcoma, oral squamous cell carcinoma breast and lung cancers. Unlike Lactose Dehyrogenase A, LDHB mutations are not linked to any disease

Alternate Names

Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5

Entrez Gene IDs

3945 (Human); 16832 (Mouse); 24534 (Rat)

Gene Symbol

LDHB

UniProt

P07195

Additional Lactate Dehydrogenase B/LDHB Products

Product Documents for Human Lactate Dehydrogenase B/LDHB Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Lactate Dehydrogenase B/LDHB Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Lactate Dehydrogenase B/LDHB Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9205

Key Product Details

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Product Specifications

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Scientific Data Images for Human Lactate Dehydrogenase B/LDHB Antibody

Detection of Human Lactate Dehydrogenase B/LDHB by Western Blot.

Detection of Human Lactate Dehydrogenase B/LDHB by Simple WesternTM.