Human Lactate Dehydrogenase B/LDHB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9205
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2057D
Product Specifications
Immunogen
E. coli-derived recombinant human LDHB
Met1-Val93
Accession # P07195
Met1-Val93
Accession # P07195
Specificity
Detects human LDHB in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Lactate Dehydrogenase B/LDHB Antibody
Detection of Human Lactate Dehydrogenase B/LDHB by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Human Lactate Dehydrogenase B/LDHB by Simple WesternTM.
Simple Western shows lysates of Exosome Standard (LNCaP) (NBP3-11687) and PC-3 human prostate cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 38 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.Lactate Dehydrogenase B/LDHB in Human Liver.
Lactate Dehydrogenase B/LDHB was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Lactate Dehydrogenase B/LDHB Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Knockout Validated
Lactate
Dehydrogenase B/LDHB is specifically detected in HEK293T human embryonic kidney parental cell line but
is not detectable in Lactate Dehydrogenase B/LDHB knockout HEK293Tcell line.
Simple Western
10 µg/mL
Sample: Exosome Standard (LnCAP) (Catalog # NBP3-11687) and PC-3 human prostate cancer cell line
Sample: Exosome Standard (LnCAP) (Catalog # NBP3-11687) and PC-3 human prostate cancer cell line
Western Blot
0.05 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB9205 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitue at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lactate Dehydrogenase B/LDHB
Alternate Names
Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5
Gene Symbol
LDHB
UniProt
Additional Lactate Dehydrogenase B/LDHB Products
Product Documents for Human Lactate Dehydrogenase B/LDHB Antibody
Product Specific Notices for Human Lactate Dehydrogenase B/LDHB Antibody
For research use only
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