Human Lamin B1 Antibody

Detection of Human Lamin B1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lamin B1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Lamin B1 in Human Liver.

Lamin B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Lamin B1 in HeLa Human Cell Line.

Lamin B1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.