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Human LAP (TGF-beta 1) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7754

R&D Systems, part of Bio-Techne
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MAB7754-100
MAB7754-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1015016

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LAP (TGF‑ beta1)
Met1-Ser390
Accession # P01137

Specificity

Detects human LAP (TGF‑ beta1) in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LAP (TGF-beta 1) Antibody

Human LAP (TGF-beta 1) Antibody in ELISA Standard Curve.

Human LAP (TGF‑ beta1) ELISA Standard Curve.

Recombinant Human LAP (TGF-beta 1) protein was serially diluted 2-fold and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human LAP (TGF-beta 1) Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human LAP (TGF-beta 1) DuoSet ELISA Kit (Catalog # DY246) for convenient development of a sandwich ELISA or the Human LAP (TGF-beta 1) Quantikine ELISA Kit (Catalog # DLAP00) for a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta1, TGF‑ beta2, TGF‑ beta1.2, TGF‑ beta3, TGF‑ beta4, and TGF‑ beta5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Alternate Names

LAP (TGFbeta 1)

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

UniProt

Additional LAP (TGF-beta 1) Products

Product Documents for Human LAP (TGF-beta 1) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LAP (TGF-beta 1) Antibody

For research use only

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