Human LAT Antibody

LAT (Linker for activation of T cells; also pp36) is a 36‑38 kDa type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TcR, GPVI, and Fc epsilonRI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLC gamma1, SLP76 and Grb2. Human LAT is 262 amino acids (aa) in length. It contains a four aa extracellular segment with a 235 aa cytoplasmic domain (aa 28‑262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36 aa upstream of the standard site that may be accompanied by a deletion of aa 114‑142. There is also a deletion of aa 88‑97, and three substitutions: two aa for aa 215‑262, 38 aa for aa 55‑262, and 50 aa for aa 35‑262. Over aa 30‑113, human LAT shares 73% aa identity with mouse LAT.