Human peripheral blood monoctyes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 Monoclonal Antibody (Catalog # MAB099) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Leukotriene B4 R1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9).

References

Samuelsson, B. et al. (1987) Science 237:1171.
Brom, J. and W. Konig (1989) Immunology 68:479.
Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
Dasari, V.R. et al. (2000) Immunopharm. 48:157.
Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
Yokomizo, T. et al. (1997) Nature 387:620.
Kato, K. et al. (2000) J. Exp. Med. 192:413.
Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.

Long Name

Leukotriene B4 Receptor 1

Alternate Names

BLT1, Leukotriene B4R1, LTB4R, LTB4R1

Entrez Gene IDs

1241 (Human)

Gene Symbol

LTB4R

Additional Leukotriene B4 R1 Products

Product Documents for Human Leukotriene B4 R1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Leukotriene B4 R1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Leukotriene B4 R1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB099

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Leukotriene B4 R1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Leukotriene B4 R1 Antibody

Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry.