Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

FRET

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 203/14F11

View all Leukotriene B4 R1 Antibodies »

Product Specifications

Immunogen

HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1

Specificity

Recognizes human Leukotriene B4 R1 (5).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Leukotriene B4 R1 Fluorescein-conjugated Antibody

Detection of Leukotriene B4 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB099F) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Leukotriene B4 R1 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 1 review rated 5 using FAB099F in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). Enhanced LTB4 production and engagement of the BLT receptors can be important in allergic and inflammatory diseases such as asthma (10), allergic encephalomyelitis (11), endotoxic shock (12), ischemia (12), psoriasis (13), rheumatoid arthritis (14) and inflammatory bowel disease (15). In addition, it has been reported that BLT1 can function as an additional co-receptor for HIV infection of CD4+ T cells (16, 17). Investigations into the mechanisms and potential inhibitors of LTB4 binding to its’ receptors may provide insight into possible treatment modalities for a number of inflammatory disorders.

References

  1. Samuelsson, B. et al. (1987) Science 237:1171.
  2. Brom, J. and W. Konig (1989) Immunology 68:479.
  3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
  4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
  5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
  6. Yokomizo, T. et al. (1997) Nature 387:620.
  7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
  8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
  9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
  10. Turner, C.R. et al. (1996) J. Clin. Invest. 97:381.
  11. Gladue, R.P. et al. (1996) J. Exp. Med. 183:1893.
  12. Lefer, A.M. (1986) Biochem. Pharmacol. 35:123.
  13. Iverson, L. et al. (1997) Skin Pharmacol. 10:169.
  14. Griffiths, R.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:517.
  15. Sharon, P. and W.F. Stenson (1984) Gastroenterology 86:453.
  16. Owman, C. et al. (1996) Proc. Natl. Acad. Sci. USA 95:9530.
  17. Martin, V. et al. (1999) J. Biol. Chem. 274:8597.

Long Name

Leukotriene B4 Receptor 1

Alternate Names

BLT1, Leukotriene B4R1, LTB4R, LTB4R1

Entrez Gene IDs

1241 (Human)

Gene Symbol

LTB4R

Additional Leukotriene B4 R1 Products

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Certificate of Analysis

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Product Specific Notices for Human Leukotriene B4 R1 Fluorescein-conjugated Antibody

For research use only

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