Human Lgr4/GPR48 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7750S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 852229
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Lgr4/GPR48
Met1-Asp951
Accession # Q9BXB1
Met1-Asp951
Accession # Q9BXB1
Specificity
Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Lgr4/GPR48 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human Lgr4/GPR48 and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Lgr4/GPR48
Lgr4 (Leucine-rich repeat GPR 4), also known as GPR48 (G-Protein-coupled Receptor 48), is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein-independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr4 binds and forms complexes with R-Spondins, Frizzled Wnt receptors and LRP Wnt co-receptors (5). It acts at least in part by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta-catenin (3, 4). Human Lgr4 cDNA encodes 951 amino acids (aa), including a long N-terminal Extracellular Domain (ECD, aa 25-544) with 16-17 LRR domains that mediate ligand interaction (1). The LRR-containing ECD of human Lgr4 shares 93% aa sequence identity with mouse, rat and bovine Lgr4, and 50-60% aa identity with human Lgr5 and Lgr6. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7-9). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over-expressed in carcinomas and may promote invasiveness and metastasis by down-regulating p27Kip1 expression (10).
References
- Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
- Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
- Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
- Glinka, A. et al. (2011) EMBO Rep. 12:1055.
- de Lau, W. et al. (2011) Nature 476:293.
- Ruffner, H. et al. (2012) PLoS ONE 7:e40975.
- Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.
- Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.
- Song, H. et al. (2008) J. Biol. Chem. 283:36687.
- Gao, Y. et al. (2006) Cancer Res. 66:11623.
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 4
Alternate Names
GPR48
Gene Symbol
LGR4
UniProt
Additional Lgr4/GPR48 Products
Product Documents for Human Lgr4/GPR48 Alexa Fluor® 750-conjugated Antibody
Product Specific Notices for Human Lgr4/GPR48 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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