Human LIMD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8494
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 919330
Product Specifications
Immunogen
E. coli-derived recombinant human LIMD1
Met1-Val143
Accession # Q9UGP4
Met1-Val143
Accession # Q9UGP4
Specificity
Detects human LIMD1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human LIMD1 Antibody
Detection of Human LIMD1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LIMD1 Monoclonal Antibody (Catalog # MAB8494) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LIMD1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LIMD1 in HeLa Human Cell Line.
LIMD1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LIMD1 Monoclonal Antibody (Catalog # MAB8494) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LIMD1 in Human Breast Cancer Tissue.
LIMD1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human LIMD1 Monoclonal Antibody (Catalog # MAB8494) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human LIMD1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and U2OS human osteosarcoma cell line
Sample: Jurkat human acute T cell leukemia cell line and U2OS human osteosarcoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIMD1
Long Name
LIM Domains Containing 1
Alternate Names
LIM domain-containing protein 1, LIM domains containing 1
Gene Symbol
LIMD1
UniProt
Additional LIMD1 Products
Product Documents for Human LIMD1 Antibody
Product Specific Notices for Human LIMD1 Antibody
For research use only
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