Human LRIG2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB19411
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 990332
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LRIG-2
Gly41-Thr805
Accession # O94898
Gly41-Thr805
Accession # O94898
Specificity
Detects human LRIG2 in immunocytochemistry and immunohistochemistry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human LRIG2 Antibody
LRIG2 in WM‑115 Human Cell Line.
LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LRIG2 in Human Kidney.
LRIG2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
LRIG2 in Human Squamous Cell Carcinoma.
LRIG2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human LRIG2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed WM-115 human malignant melanoma cell line
Sample: Immersion fixed WM-115 human malignant melanoma cell line
Immunohistochemistry
1.7-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and human squamous cell carcinoma
Sample: Immersion fixed paraffin-embedded sections of human kidney and human squamous cell carcinoma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB19411 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRIG2
Long Name
Leucine-rich Repeats and Immunoglobulin-like Domains 2
Alternate Names
KIAA0806, LIG2, UFS2
Gene Symbol
LRIG2
UniProt
Additional LRIG2 Products
Product Documents for Human LRIG2 Antibody
Product Specific Notices for Human LRIG2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...