Human LRRC3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5039
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 532820
Product Summary for Human LRRC3 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRC3
Cys33-Asp204
Accession # Q9BY71
Cys33-Asp204
Accession # Q9BY71
Specificity
Detects human LRRC3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LRRC3 Antibody
LRRC3 in Human Brain.
LRRC3 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human LRRC3 Monoclonal Antibody (Catalog # MAB5039) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human LRRC3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC3
LRRC3 (Leu-rich repeat/LRR-containing protein 3) is a 25 kDa (predicted) member of the Leu-rich repeat protein superfamily. Little is known about the protein except for the fact that it shows widespread expression. Human LRRC3 is a protein that is 225 amino acids (aa) in length. It contains three LRRs between aa 63‑86, aa 87‑110 and aa 112‑135. There is one potential splice variant that shows a deletion of aa 129‑248. Over aa 33‑204, human LRRC3 is 83% and 81% aa identical to mouse and canine LRRC3, respectively.
Long Name
Leucine-rich Repeat Containing 3
Alternate Names
C21orf102, chromosome 21 open reading frame 102, leucine rich repeat containing 3, leucine-rich repeat-containing protein 3, LRRC3A
Gene Symbol
LRRC3
UniProt
Additional LRRC3 Products
Product Documents for Human LRRC3 Antibody
Product Specific Notices for Human LRRC3 Antibody
For research use only
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