Human LRTM1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10046
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2381D
Product Specifications
Immunogen
Synthetic peptide containing human LRTM1
Accession # Q9HBL6
Accession # Q9HBL6
Specificity
Detects human LRTM1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human LRTM1 Antibody
Detection of Human LRTM1 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LRTM1 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRTM1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LRTM1 in HEK293 Human Cell Line Transfected with Human LRTM1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human LRTM1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
LRTM1 in TK-6 and A549 Human Cell Lines.
LRTM1 was detected in immersion fixed TK-6 human lymphoblastoid cell line (left panel; positive stain) and A549 human lung carcinoma cell line (right panel; negative stain) using Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human LRTM1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human LRTM1 and EGFP
Sample: HEK293 Human Cell Line Transfected with Human LRTM1 and EGFP
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed TK-6 human lymphoblastoid cell line
Sample: Immersion fixed TK-6 human lymphoblastoid cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Western Blot
2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human LRTM1 and eGFP
Sample: HEK293T human embryonic kidney cell line transfected with human LRTM1 and eGFP
Reviewed Applications
Read 1 review rated 5 using MAB10046 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRTM1
Long Name
Leucine-rich Repeats and Transmembrane Domains 1
Alternate Names
HT017, leucine-rich repeat and transmembrane domain-containing protein 1, leucine-rich repeats and transmembrane domains 1
Gene Symbol
LRTM1
UniProt
Additional LRTM1 Products
Product Documents for Human LRTM1 Antibody
Product Specific Notices for Human LRTM1 Antibody
For research use only
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