Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6746
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human KDM3A
Pro844-Glu968
Accession # Q9Y4C1
Pro844-Glu968
Accession # Q9Y4C1
Specificity
Detects human KDM3A in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reactivity with recombinant human (rh) KDM4A is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody
Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human KDM3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6746) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KDM3A at approximately 150 kDa (as indicated), the band at approximately 70kDa represents the product of degradation or processing. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: 293T human embryonic kidney cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: 293T human embryonic kidney cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lysine (K)-specific Demethylase 3A/KDM3A
JMJD1A (Jumonji [Japanese for "cruciform/cross"]/ARID domain-containing protein 1A; also KDM3A, JHDM2A, and TSGA) is a 140-150 kDa member of the JHDM2 histone demethylase family of proteins. It is induced by hypoxia, and expressed in spermatids, smooth muscle cells, mammary epithelium, hepatocytes, and renal tubular epithelium. JMJD1A is reported to demethylate di- and mono-methylated Lys9 on histone H3. It has no activity on trimethylated Lys9. JMJD1A is both cytoplasmuc and nuclear, and serves as a coactivator for the AR. Human JMJD1A is 1321 amino acids (aa) in length. It contains one C6-type zinc-finger motif (aa 662-687) and a jumonji C type catalytic domain (aa 1151-1264). There is one phosphorylation site at Ser445. There is one potential splice variants that shows a 13 aa substitution for aa 699-1321. Over aa 810-968, human JMJD1A shares 94% aa identity with mouse JMJD1A.
Alternate Names
JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA
Gene Symbol
KDM3A
UniProt
Additional Lysine (K)-specific Demethylase 3A/KDM3A Products
Product Documents for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody
Product Specific Notices for Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody
For research use only
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