Human MAP4K5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8007
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MAP4K5
Lys395-Asn484 (Asn473Lys)
Accession # Q9Y4K4
Lys395-Asn484 (Asn473Lys)
Accession # Q9Y4K4
Specificity
Detects human MAP4K5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human MAP4K5 Antibody
Detection of Human MAP4K5 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MAP4K5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAP4K5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MAP4K5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line
Sample: 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line
Reviewed Applications
Read 1 review rated 5 using AF8007 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAP4K5
MAP4K5 (MAP Kinase Kinase Kinase Kinase 5; also known as KHS, MEKKK5, and GCKR) is a 94-96 kDa member of the STE (STErile 20 protein) subfamily, STE Ser/Thr protein kinase family, protein kinase superfamily of enzymes. It is ubiquitously expressed, and serves as an "intermediary" between GPCRs and cytosolic MAP kinases. It also interacts with Crk (CT10 Regulator of Kinase)-related adaptor molecules, facilitating the formation of signaling complexes around Tyr phosphorylated molecules. And finally, it represents an upstream component of a TNFR1 signaling pathway that requires activation of stress-activated protein kinases. Human MAP4K5 is 846 amino acids 9aa) in length. It contains a 258 aa kinase domain (aa 20-277) that is coupled to a 316 aa CNH (citron homology) domain (aa 506-826) which typically binds GTPases. There are two isoform variants. One shows a deletion of both Pro481 and Glu569, while another possesses a substitution of 16 aa for aa 569-846. Over aa 395-484, human MAP4K5 shares 83% aa sequence identity with mouse MAP4K5.
Long Name
Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase
Alternate Names
GCKR, KHS1, MAPKKKK5
Gene Symbol
MAP4K5
UniProt
Additional MAP4K5 Products
Product Documents for Human MAP4K5 Antibody
Product Specific Notices for Human MAP4K5 Antibody
For research use only
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