Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2783C

View all mCherry Antibodies »

Product Specifications

Immunogen

Human mCherry synthetic peptide

Specificity

Detects human mCherry in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human mCherry Antibody

Detection of Human mCherry by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mVISTA. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human mCherry Monoclonal Antibody (Catalog # MAB11041) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for mCherry at approximately 30-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

mCherry in HEK293 Human Cell Line Transfected with mCherry.

mCherry was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with mCherry using Rabbit Anti-Human mCherry Monoclonal Antibody (Catalog # MAB11041) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of mCherry in HEK293 Human Cell Line transfected with mCherry by Flow Cytometry.

HEK293 human cell line transfected with mCherry (filled histogram) or EGFP (open histogram) was stained with Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041), followed by Allophycocyanin-conjugated Anti-Rabbit IgG F(ab')2Secondary Antibody (F0111). Gates were set based on isotype control antibody (MAB1050, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
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Applications for Human mCherry Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with mCherry

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mCherry

Simple Western

25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with mVISTA

Western Blot

0.25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with mVISTA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mCherry

mCherry is a member of the mFruits family of monomeric red fluorescent proteins. It was derived from DsRed of Discoscoma sea anemones. mFruit are second-generation monomeric red fluorescent proteins that have improved brightness and photostability compared to first-generation proteins. The gene for mCherry is 711bp long and the protein is made up of 236 residues with a mass of 76.722 kDa. mCherry is used in fluorescence microscopy as an intracellular probe where constitutive gene expression is desired and other experimental approaches require coordinated control of multiple genes.

References

  1. Shaner, N.C. Campbell, R.E. Steinbach, P.A. Giepmans B.N.G. Palmer A.E. Tsien, R Y. "Improved Monomeric Red, Orange and Yellow Fluorescent Proteins Derived from Discomsoma Sp. Red Fluorescent Protein" Nature Biotechnology. 22(12):1567. 2004 Nov. 21.
  2. Shu, X. Shaner N C. Yarbrough, C.A. Tsien, R Y. Remington S.J. "Novel Chromophores and Buried Charges Control Color in mFruits" Biochemistry. 45(32):9639. 2006 Aug.
  3. Gebhardt, M.J. Jacobson, R.K. Shuman, H. "Seeing Red; The Development of pON mCherry, a Broad-host Range Constitutive Expression Plasmid for Gram-negative Bacteria" POS ONE. 12(3):e173116. 2017 Mar. 3.

Long Name

mCherry

Alternate Names

DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

Additional mCherry Products

Product Documents for Human mCherry Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human mCherry Antibody

For research use only

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