Human MCHR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB79382
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1032150
Product Specifications
Immunogen
E. coli-derived human MCHR1
Met1-Pro113
Accession # Q99705
Met1-Pro113
Accession # Q99705
Specificity
Detects human MCHR1 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MCHR1 Antibody
MCHR1 in IMR32 Human Cell Line.
MCHR1 was detected in immersion fixed IMR32 human neuroblastoma cell line (left panel; positive staining) and RT-4 human urinary bladder transitional cell papilloma cell line (right panel; negative control) using Mouse Anti-Human MCHR1 Monoclonal Antibody (Catalog # MAB79382) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
MCHR1 in Human Brain (Hypothalamus).
MCHR1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human MCHR1 Monoclonal Antibody (Catalog # MAB79382) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human MCHR1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed IMR32 human neuroblastoma cell line
Sample: Immersion fixed IMR32 human neuroblastoma cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Reviewed Applications
Read 1 review rated 5 using MAB79382 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCHR1
Long Name
Melanin-concentrating Hormone Receptor 1
Alternate Names
GPR24, MCH1R, SLC1
Gene Symbol
MCHR1
UniProt
Additional MCHR1 Products
Product Documents for Human MCHR1 Antibody
Product Specific Notices for Human MCHR1 Antibody
For research use only
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