Human MCM7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9217
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2068B
Product Specifications
Immunogen
E. coli-derived recombinant human MCM7
Gly188-Ala328
Accession # P33993
Gly188-Ala328
Accession # P33993
Specificity
Detects human MCM7 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human MCM7 Antibody
Detection of Human MCM7 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MCM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MCM7 in HeLa Human Cell Line.
MCM7 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MCM7 in Human Mesothelioma Tissue.
MCM7 was detected in immersion fixed paraffin-embedded sections of human mesothelioma tissue using Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human MCM7 Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human mesothelioma tissue
Sample: Immersion fixed paraffin-embedded sections of human mesothelioma tissue
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Sample: HeLa human cervical epithelial carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line
Reviewed Applications
Read 1 review rated 5 using MAB9217 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCM7
MCM7/minichromosome maintenance complex component 7, is a highly conserved mini-chromosome maintenance protein, with ATP binding, DNA helicase activity and single-stranded DNA binding. MCM7 forms a hexameric protein complex with MCM2, 4 and 6 proteins that has DNA helicase activity essential for the initiation of gene replication. MCM7 also binds to RAD17 to mediate ATR recruitment to damaged DNA. MCM7 expression is repressed in quiescent cells but induced in late G1 to S phase by growth factor stimulation. It is up-regulated in many cancers including colorectal , T cell lymphomas, glioblastoma, and has prognostic value for hepatocellular carcinoma and gastric adenocarcinoma.
Long Name
Minichromosome Maintenance Protein 7
Alternate Names
CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1
Gene Symbol
MCM7
UniProt
Additional MCM7 Products
Product Documents for Human MCM7 Antibody
Product Specific Notices for Human MCM7 Antibody
For research use only
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