Mouse Anti-Human MCT1/SLC16A1 Monoclonal Antibody (Catalog # MAB8275) was detected in (A) HEK293 human embryonic kidney cell line transfected with human MCT1/SLC16A1 and eGFP or (B) Irrelevant transfectants followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MCT1/SLC16A1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human MCT1/SLC16A1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCT1/SLC16A1

Solute Carrier, family 16, member 1 (SLC16A1) encodes Monocarboxylic Acid Transporter 1 (MCT1). MCT1 is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. MCT1 has been found to be important in lactate uptake in cancer cells, and blockade of MCT1 may inhibit lactate-induced HIF-1 activation (1).

References

Sonveaux, P. et al (2012) PLoS ONE 7:e33418.

Long Name

Monocarboxylic Acid Transporter 1 (Solute Carrier Family 16 Member 1)

Alternate Names

HHF7, SLC16A1

Entrez Gene IDs

6566 (Human); 20501 (Mouse); 25027 (Rat)

Gene Symbol

SLC16A1

UniProt

P53985

Additional MCT1/SLC16A1 Products

Product Documents for Human MCT1/SLC16A1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MCT1/SLC16A1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MCT1/SLC16A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8275

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MCT1/SLC16A1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MCT1/SLC16A1 Antibody

Detection of MCT1/SLC16A1 in HEK293 Human Cell Line Transfected with Human MCT1/SLC16A1 and eGFP by Flow Cytometry.