HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB32652F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Mesothelin Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

AAH09272

Additional Mesothelin Products

Product Documents for Human Mesothelin Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Mesothelin Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Mesothelin Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB32652F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Mesothelin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Mesothelin Fluorescein-conjugated Antibody

Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry.