Human mGluR8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5277
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 476410
Product Summary for Human mGluR8 Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human mGluR8
Gln34-Ser514 (predicted)
Accession # O00222
Gln34-Ser514 (predicted)
Accession # O00222
Specificity
Detects human mGluR8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human mGluR1, 2, 3, 4, 5, or 7 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human mGluR8 Antibody
mGluR8 in Human Brain.
mGluR8 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human mGluR8 Monoclonal Antibody (Catalog # MAB5277) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human mGluR8 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Western Blot
1 µg/mL
Sample: Human mGluR8
Sample: Human mGluR8
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB5277 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: mGluR8
mGluR8 is a Group III metabotropic glutamate receptor. These 7-transmembrane glycoproteins are negatively coupled to adenylate cyclase and expressed presynaptically throughout the brain. mGluR8 shows pronounced expression in the dentate gyrus and CA3 regions of the hippocampus, and is thought to play a role in memory formation. Two splice forms, A and B, differ only in the C-terminal 16 amino acids (aa 893‑909), while splice form C diverges at aa 454 and is truncated prior to the first transmembrane segment. Within the N-terminal extracellular region used as an immunogen (aa 34‑514), human, mouse and rat mGluR8 share 98%‑99% aa identity.
Long Name
Metabotropic Glutamate Receptor 8
Alternate Names
GPRC1H, GRM8
Gene Symbol
GRM8
UniProt
Additional mGluR8 Products
Product Documents for Human mGluR8 Antibody
Product Specific Notices for Human mGluR8 Antibody
For research use only
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