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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 753002

Product Specifications

Immunogen

E. coli-derived recombinant human MINA
Met1-Gly192
Accession # Q8IUF8

Specificity

Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MINA Alexa Fluor® 488-conjugated Antibody

Detection of MINA antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of MINA in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7476G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human MINA Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MINA

MINA (Myc-induced Nuclear Antigen), also known as Mina53, is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and particularly nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, select epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R, IL-4, and Relm beta, the latter through the influence of TGF-beta. It may exert its regulatory activity through an intrinsic histone demethylase function. Human MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 52-364) that contains a JmjC domain (aa 139-271). There are three potential isoform variants that show either a seven aa substitution for aa 255‑261, an 18 aa substitution for aa 263-465, or a deletion of Glu297. Over aa 2-192, human MINA shares 82% aa sequence identity with mouse MINA.

Long Name

Myc-induced Nuclear Antigen

Alternate Names

MDIG, Mina53, NO52

Entrez Gene IDs

84864 (Human); 67014 (Mouse); 266670 (Rat)

Gene Symbol

RIOX2

UniProt

Additional MINA Products

Product Documents for Human MINA Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MINA Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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