Human MMP-8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF908
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-8
Phe21-Gly467
Accession # AAZ38714
Phe21-Gly467
Accession # AAZ38714
Specificity
Detects human MMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse MMP-8 and recombinant rat MMP-8 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MMP-8 Antibody
MMP‑8 in Human Breast.
MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP‑8 in Jurkat Human Cell Line.
MMP-8 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human MMP-8 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast array
Sample: Immersion fixed paraffin-embedded sections of human breast array
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-8 (Catalog # 908-MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-8 (Catalog # 908-MP), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human MMP-8 Western Blot Standard (Catalog # WBC017)
Sample: Recombinant Human MMP-8 Western Blot Standard (Catalog # WBC017)
Reviewed Applications
Read 1 review rated 3 using AF908 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-8
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta-casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.
Long Name
Matrix Metalloproteinase 8
Alternate Names
Collagenase 2, MMP8
Gene Symbol
MMP8
UniProt
Additional MMP-8 Products
Product Documents for Human MMP-8 Antibody
Product Specific Notices for Human MMP-8 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...