Human MMP-8 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF908
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
ELISA Detection
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-8
Phe21-Gly467
Accession # AAZ38714
Phe21-Gly467
Accession # AAZ38714
Specificity
Detects human MMP‑8 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) rhMMP-1, rhMMP-2, rhMMP-9, rhMMP-13, rhMMP-7, rhMMP-10 and rhMMP-12 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MMP-8 Biotinylated Antibody
MMP‑8 in Human Breast Cancer Tissue.
MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human MMP-8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MMP-8 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human MMP‑8 (Catalog # 908-MP)
Sample: Recombinant Human MMP‑8 (Catalog # 908-MP)
Human MMP-8 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-8
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta-casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP-8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.
Long Name
Matrix Metalloproteinase 8
Alternate Names
Collagenase 2, MMP8
Gene Symbol
MMP8
UniProt
Additional MMP-8 Products
Product Documents for Human MMP-8 Biotinylated Antibody
Product Specific Notices for Human MMP-8 Biotinylated Antibody
For research use only
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