Human/Mouse Acetyl Histone H4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5215
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Tetrahymena pyriformis acetylated Histone H4 synthetic peptide
AGGKGGKGMGKVGAKRHSK
Accession # P02310
AGGKGGKGMGKVGAKRHSK
Accession # P02310
Specificity
Detects immunogen in direct ELISAs and human Acetyl Histone H4 in Western blots. Reactivity with non-acetylated peptide was removed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse Acetyl Histone H4 Antibody
Detection of Human Acetyl Histone H4 by Western Blot.
Western blot shows lysates of Tera-2 human embryonic lung carcinoma cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Acetyl Histone H4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Histone H4 in NTera‑2 Human Cell Line.
Histone H4 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse Acetyl Histone H4 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line
Western Blot
1 µg/mL
Sample: Tera‑2 human embryonic lung carcinoma cell line, Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line
Sample: Tera‑2 human embryonic lung carcinoma cell line, Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone H4
Histone 4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. It is ubiquitously expressed, with H4 initially synthesized and deposited in replicating chromatin during S phase. H4 undergoes multiple posttranslational modifications. At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation. As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively. H4 is also known to be phosphorylated on Ser2 and 48, plus Tyr52 and 89, and acetylated on at least nine sites. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4, and 78% aa identical to Tetrahymena histone 4 over aa 2-20 used for immunization.
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4-16
UniProt
Additional Histone H4 Products
Product Documents for Human/Mouse Acetyl Histone H4 Antibody
Product Specific Notices for Human/Mouse Acetyl Histone H4 Antibody
For research use only
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