Human/Mouse Adropin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9690
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 976401
Product Specifications
Immunogen
Synthetic peptide containing human Adropin
Accession # Q6UWT2
Accession # Q6UWT2
Specificity
Detects human Adropin in direct ELISAs. Detects human and mouse Adropin in immunocytochemistry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Adropin Antibody
Adropin in SH‑SY5Y Human Cell Line.
Adropin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Adropin Monoclonal Antibody (Catalog # MAB9690) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Adropin in Mouse Kidney.
Adropin was detected in perfusion fixed frozen sections of mouse kidney using Mouse Anti-Human Adropin Monoclonal Antibody (Catalog # MAB9690) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Adropin in Human Brain Cortex.
Adropin was detected in Immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human/Mouse Adropin Monoclonal Antibody (Catalog # MAB9690) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes.Applications for Human/Mouse Adropin Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample:
Sample:
Immersion fixed SH-SY5Y human neuroblastoma cell line
Immunohistochemistry
5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney
Sample: Perfusion fixed frozen sections of mouse kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adropin
Adropin (from the gene energy homeostasis-associated protein, ENHO) is a 76 amino acid peptide hormone encoded by the gene ENHO. Adropin levels are regulated by nutrition; a high fat diet in lean C57BL/6J mice produced rapid increase in ENHO expression. It is preferentially expressed in brain and has cardiovascular and metabolic roles in the periphery. Adropin plays a role in metabolic homeostasis, fatty acids metabolism control, insulin resistance prevention, Dyslipidemia and impaired glucose tolerance. Adropin level is thought to be a factor in obesity because it plays a role in regulating carbohydrate, lipid and protein metabolisms by moderating glucose-mediated insulin release. High plasma levels of Adropin are associated with heart failure and body mass index. Adropin has also been shown to preserve the blood brain barrier and improve neuronal function after intracerebral hemorrhage in mice.
Alternate Names
C9orf165, ENHO, UNQ470
Gene Symbol
ENHO
UniProt
Additional Adropin Products
Product Documents for Human/Mouse Adropin Antibody
Product Specific Notices for Human/Mouse Adropin Antibody
For research use only
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