Human/Mouse Aminopeptidase PILS/ARTS1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2334
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6H9
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 9-derived recombinant human ARTS1
Accession # Q9NZ08
Accession # Q9NZ08
Specificity
Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse Aminopeptidase PILS/ARTS1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse Aminopeptidase PILS/ARTS1 Antibody
Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Aminopeptidase PILS/ARTS1 by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 114 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Aminopeptidase PILS/ARTS1 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Sample: A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Western Blot
2 µg/mL
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 4 using MAB2334 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase PILS/ARTS1
Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Alternate Names
A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP
Gene Symbol
ERAP1
UniProt
Additional Aminopeptidase PILS/ARTS1 Products
Product Documents for Human/Mouse Aminopeptidase PILS/ARTS1 Antibody
Product Specific Notices for Human/Mouse Aminopeptidase PILS/ARTS1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...