Human/Mouse BACE-1 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF931
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BACE‑1 Ectodomain
Thr22-Tyr460
Accession # P56817
Thr22-Tyr460
Accession # P56817
Specificity
Detects human and mouse BACE‑1 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) ADAM8, recombinant mouse ADAM9, rhADAM15, and rhTACE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human/Mouse BACE-1 Ectodomain Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human BACE‑1 (Catalog # 931‑AS)
Recombinant Mouse BACE‑1 (Catalog # 2976‑AS)
Sample: Recombinant Human BACE‑1 (Catalog # 931‑AS)
Recombinant Mouse BACE‑1 (Catalog # 2976‑AS)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-1
BACE-1 is an aspartic protease and an integral membrane protein (1 - 5). BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein beta site in the brain to generate the amyloid beta peptide. Because the amyloid beta peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 - 5.5), consistent with its proposed function in an acidic intracellular compartment.
References
- Ermolieff, J. et al. (2000) Biochemistry 39:12450.
- Lin, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1456.
- Sinha, S. et al. (1999) Nature 402:537.
- Vassar, R. et al. (1999) Science 286:735.
- Yan, R. et al. (1999) Nature 402:533.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Alternate Names
BACE1
Gene Symbol
BACE1
UniProt
Additional BACE-1 Products
Product Documents for Human/Mouse BACE-1 Ectodomain Antibody
Product Specific Notices for Human/Mouse BACE-1 Ectodomain Antibody
For research use only
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