Human/Mouse Bax Minus C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB883
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 127602
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Bax minus the carboxyl terminal
Specificity
Detects human and mouse full-length Bax in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse Bax Minus C-Terminus Antibody
Detection of Human/Mouse Bax Minus C-Terminus by Western Blot.
Western blot shows lysates of L-929 mouse fibroblast cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1.0-2.0 µg/mL of Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Bax in A549 Human Cell Line.
Bax was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Bax in RAW264.7 cells.
Bax was detected in immersion fixed RAW264.7 cells using Mouse Anti-Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse Bax Minus C-Terminus Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Western Blot
1.0-2.0 µg/mL
Sample: L-929 mouse fibroblast cell line and THP-1 human acute monocytic leukemia cell line
Sample: L-929 mouse fibroblast cell line and THP-1 human acute monocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Bax
Long Name
Bcl Associated X Protein
Alternate Names
apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4
Gene Symbol
BAX
Additional Bax Products
Product Documents for Human/Mouse Bax Minus C-Terminus Antibody
Product Specific Notices for Human/Mouse Bax Minus C-Terminus Antibody
For research use only
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