Human/Mouse COX4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6980

R&D Systems, part of Bio-Techne
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MAB6980
MAB6980-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 673803

View all COX4 Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human COX4-I2
Asn39-Lys171
Accession # Q96KJ9

Specificity

Detects human COX4-I2 in direct ELISAs. In Western blots, 100% cross-reactivity with recombinant human (rh) COX4-I1 andno cross-reactivity with rhCOX-1 or rhCOX-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse COX4 Antibody

Detection of Human and Mouse COX4 antibody by Western Blot.

Detection of Human and Mouse COX4 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX4 Monoclonal Antibody (Catalog # MAB6980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For addtional reference, recombinant human COX4-I1 (5 ng/lane) and recombinant human COX4-I2 (2.5 ng/lane) were included. A specific band was detected for COX4 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
COX4 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

COX4 in NIH‑3T3 Mouse Cell Line.

COX4 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
COX4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

COX4 in HeLa Human Cell Line.

COX4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse COX4 Monoclonal Antibody (Catalog # MAB6980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse COX4 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, recombinant human COX4-I1 and recombinant human COX4-I2

Reviewed Applications

Read 1 review rated 5 using MAB6980 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX4

COX4-I2 (Cytochrome c oxidase subunit 4) is a 21-22 kDa member of the Cytochrome c oxidase IV family of proteins and is predominantly expressed in lung. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from Cytochrome c to dioxygen. COX4-I2 is induced following blockade of mitochondrial respiration, leading to increased production of reactive oxygen species and necrosis. The ubiquitously expressed COX4-I1 is the product of a different gene and shares only 50% aa identity with COX4-I2. Within aa 39-171 (the oxidase domain), human COX4-I2 shares 84% and 81% aa sequence identity with mouse and rat COX4-I2, respectively.

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Entrez Gene IDs

84701 (Human); 84682 (Mouse); 84683 (Rat)

Gene Symbol

COX4I2

UniProt

Q96KJ9

Additional COX4 Products

Product Documents for Human/Mouse COX4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse COX4 Antibody

For research use only

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