Human/Mouse DCBLD2/ESDN Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6269
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DCBLD2/ESDN
Gln67-Ala528
Accession # Q96PD2
Gln67-Ala528
Accession # Q96PD2
Specificity
Detects human and mouse DCBLD2/ESDN in Western blots and human DCBLD2/ESDN in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse DCBLD2/ESDN Antibody
Detection of Mouse DCBLD2/ESDN by Western Blot.
Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCBLD2/ESDN at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of DCBLD2/ESDN in NCI-H460 Human Cell Line by Flow Cytometry.
NCI-H460 human large cell lung carcinoma cell line was stained with Human/Mouse DCBLD2/ESDN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6269, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).Applications for Human/Mouse DCBLD2/ESDN Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: NCI‑H460 human large cell lung carcinoma cell line
Sample: NCI‑H460 human large cell lung carcinoma cell line
Western Blot
0.5 µg/mL
Sample: P19 mouse embryonal carcinoma cell line
Sample: P19 mouse embryonal carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DCBLD2/ESDN
DCBLD2 (discoidin, CUB, and LCCL domain containing 2), also known as ESDN (endothelial and smooth muscle cell derived neuropilin-like) and CLCP1, was identified through its up-regulation in a highly metastatic lung cancer cell line. DCBLD2 has structural similarities to neuropilins, VEGF receptors, and semaphorins, and is thought to play a role in cell motility and metastasis. SEMA4B is a receptor for DCBLD2/ESDN/CLCP1 and this interaction may be involved in regulating cancer cell metastasis.
Long Name
Discoidin, CUB and LCCL Domain Containing 2/Endothelial and Smooth Muscle Cell-derived Neuropilin-li
Alternate Names
CLCP1, ESDN
Gene Symbol
DCBLD2
UniProt
Additional DCBLD2/ESDN Products
Product Documents for Human/Mouse DCBLD2/ESDN Antibody
Product Specific Notices for Human/Mouse DCBLD2/ESDN Antibody
For research use only
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