Human/Mouse EED Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5827

R&D Systems, part of Bio-Techne
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AF5827
AF5827-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all EED Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EED
Ser2-Cys150
Accession # O75530

Specificity

Detects human and mouse EED in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse EED Antibody

Detection of Human EED antibody by Western Blot.

Detection of Human EED by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT-29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5827) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EED at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse EED Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HT-29 human colon adenocarcinoma cell line, human colon cancer tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EED

EED (Embryonic ectoderm development; also WAIT-1) is a 51 kDa member of the WD repeat ESC family of proteins. It is widely expressed, and constitutes part of both the PRC1 and PRC2 nuclear complexes. PRC1 contains EED, Suz12, RbAp46 and Ezh1 and is found in nonproliferating tissue. PRC2 differs only by the presence of Ezh2 and is found in proliferating tissues. Both complexes methylate histone H3 and act as gene repressors. Human EED is 441 amino acids in length. It contains three phosphorylation sites (Ser34; Thr55; Thr57) followed by seven WD40 domains (aa 91‑441). There are two alternate start sites, one at Met15, and another that is 94 aa upstream of the standard site, begins with a Val and generates a 69 kDa isoform. A third isoform variant shows an insertion of 25 aa after Ser323, while a fourth variant shows a premature truncation after Lys400. Human and mouse EED are identical in amino acid sequence.

Long Name

Embryonic Ectoderm Development

Alternate Names

WAIT1

Entrez Gene IDs

8726 (Human); 13626 (Mouse); 293104 (Rat)

Gene Symbol

EED

UniProt

O75530

Additional EED Products

Product Documents for Human/Mouse EED Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse EED Antibody

For research use only

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