Human/Mouse FoxP3 Antibody

R&D Systems, part of Bio-Techne | Catalog # FPK8214A

Recombinant Monoclonal Antibody. A flow cytometry staining kit containing an APC-conjugated FoxP3 monoclonal antibody (IC8214A) and FoxP3 Fixation and Permeabilization Buffers (FC012)
R&D Systems, part of Bio-Techne
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FPK8214A-025
FPK8214A-100
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1054C

View all FoxP3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxP3
Met1-Leu71

Specificity

Detects human and mouse FoxP3 in flow cytometry.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse FoxP3 Antibody

Detection of FoxP3 antibody in Human PBMC Natural Tregs antibody by Flow Cytometry.

Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 Ra (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes
Detection of FoxP3 antibody in C57BL/6 Mouse Splenocyte Natural Tregs antibody by Flow Cytometry.

Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry.

C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and (B) Rat Anti-Mouse IL-2 Ra (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB2438P) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.

Applications for Human/Mouse FoxP3 Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) and C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 to 8 °C. Do not use past expiration date. Protect from light.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for Human/Mouse FoxP3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse FoxP3 Antibody

For research use only

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