Does FoxP3 antibodies comes in lyophilized form?

Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 Ra (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.

Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry.

C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and (B) Rat Anti-Mouse IL-2 Ra (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB2438P) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 APC-conjugated Monoclonal Antibody (Catalog # IC8214A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.

Applications for Human/Mouse FoxP3 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) and C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4⁺ T cells. In human, FoxP3 is found in CD4⁺, CD8⁺ and CD4⁺CD25⁺ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Gene Symbol

FOXP3

UniProt

Q9BZS1

Additional FoxP3 Products

Product Documents for Human/Mouse FoxP3 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse FoxP3 APC-conjugated Antibody

For research use only

Citations
Reviews

Protocols
FAQs

Spectra Viewer
Flow Cytometry

Human/Mouse FoxP3 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC8214A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse FoxP3 APC-conjugated Antibody

Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry.