Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 145901

View all Frizzled-4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
Phe37-Glu180
Accession # Q61088

Specificity

Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse Frizzled-4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Frizzled-4 (Catalog # 5847-FZ)
Recombinant Mouse Frizzled-4 Fc Chimera (Catalog # 194-FZ)

Reviewed Applications

Read 4 reviews rated 4.3 using MAB194 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-4

Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89% and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).

References

  1. Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
  2. Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
  3. Xu, Q. et al. (2004) Cell 116:883.
  4. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
  5. Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
  6. Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
  7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
  8. Robitaille, J. et al. (2002) Nat. Genet. 32:326.
  9. Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.

Alternate Names

CD344, Frizzled4, FZD4

Entrez Gene IDs

8322 (Human); 14366 (Mouse)

Gene Symbol

FZD4

UniProt

Q61088

Additional Frizzled-4 Products

Product Documents for Human/Mouse Frizzled-4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Frizzled-4 Antibody

For research use only

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