Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Endotoxin Level

<0.15 EU per 1 μg of the antibody by the LAL method.

Applications for Human/Mouse Frizzled-4 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Frizzled-4 (Catalog # 5847-FZ)
Recombinant Mouse Frizzled-4 Fc Chimera (Catalog # 194-FZ)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-4

Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis.

Alternate Names

CD344, Frizzled4, FZD4

Entrez Gene IDs

8322 (Human); 14366 (Mouse)

Gene Symbol

FZD4

UniProt

Q61088

Additional Frizzled-4 Products

Product Documents for Human/Mouse Frizzled-4 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Frizzled-4 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse Frizzled-4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM194

Key Product Details

Species Reactivity

Validated:

Cited:

Applications