Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1249R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Glucagon in U2OS cells (Positive) & RT-4 cells (Negative).

Glucagon was detected in immersion fixed U2OS human osteosarcoma cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma (Negative) using Mouse Anti-Human/Mouse Glucagon NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL1249R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nucleolus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion-fixed beta TC-6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Glucagon

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Alternate Names

GCG, GRPP

Entrez Gene IDs

2641 (Human); 14526 (Mouse); 24952 (Rat)

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody

For research use only

Citations
Reviews

Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # NL1249R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Glucagon Antibodies »

Product Specifications

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody

Glucagon in beta TC-6 Mouse Cell Line.