Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # NL1249R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry
Label
NL557 (Excitation = 557 nm, Emission = 574 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 181402
Product Specifications
Specificity
Detects human and mouse Glucagon.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody
Glucagon in beta TC-6 Mouse Cell Line.
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1249R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Glucagon in U2OS cells (Positive) & RT-4 cells (Negative).
Glucagon was detected in immersion fixed U2OS human osteosarcoma cells (Positive) & absent in RT-4 human urinary bladder transitional cell papilloma (Negative) using Mouse Anti-Human/Mouse Glucagon NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL1249R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nucleolus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion-fixed beta TC-6 mouse beta cell insulinoma cell line
Sample: Immersion-fixed beta TC-6 mouse beta cell insulinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Glucagon
Additional Glucagon Products
Product Documents for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody
Product Specific Notices for Human/Mouse Glucagon NorthernLights™ NL557-conjugated Antibody
For research use only
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