Human/Mouse HIF-1 alpha/HIF1A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1935
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 241812
Product Specifications
Immunogen
E. coli-derived recombinant human HIF-1 alpha/HIF1A
Arg575-Asn826
Accession # Q16665.1
Arg575-Asn826
Accession # Q16665.1
Specificity
Recognizes human and mouse HIF-1 alpha/HIF1A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A Antibody
HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue.
HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse HIF-1 alpha/HIF1A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast and kidney cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast and kidney cancer tissue
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: CoCl2-treated MCF-7 human breast cancer cell line, fixed with paraformaldehyde, and permeabilized with saponin
Sample: CoCl2-treated MCF-7 human breast cancer cell line, fixed with paraformaldehyde, and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-1 alpha/HIF1A
The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Alternate Names
BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8
Gene Symbol
HIF1A
UniProt
Additional HIF-1 alpha/HIF1A Products
Product Documents for Human/Mouse HIF-1 alpha/HIF1A Antibody
Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A Antibody
For research use only
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