Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1935C
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 241812
Product Specifications
Immunogen
E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1
Arg575-Asn826
Accession # Q16665.1
Specificity
Recognizes human and mouse HIF-1 alpha in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line treated with 1 mM DFO overnight was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (IC002C, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.Applications for Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005), or treated with DFO and fixed and permeabilized with FlowX FoxP3/Transcription Factor Fix & Perm Kit (Catalog # FC012).
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005), or treated with DFO and fixed and permeabilized with FlowX FoxP3/Transcription Factor Fix & Perm Kit (Catalog # FC012).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HIF-1 alpha/HIF1A
The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Alternate Names
BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8
Gene Symbol
HIF1A
UniProt
Additional HIF-1 alpha/HIF1A Products
Product Documents for Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody
Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody
For research use only
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