Human/Mouse HO-1/HMOX1/HSP32 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3776
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Knockout Validated, Simple Western, Western Blot
Cited:
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HO‑1/HMOX1/HSP32
Met1-Thr261
Accession # P09601
Met1-Thr261
Accession # P09601
Specificity
Detects human and mouse HO‑1/HMOX1/HSP32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HO-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse HO-1/HMOX1/HSP32 Antibody
Detection of Human/Mouse HO‑1/HMOX1/ HSP32 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse HO-1/HMOX1/HSP32 by Simple WesternTM.
Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-1/HMOX1/HSP32 at approximately 37 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse HO-1/HMOX1/HSP32 Antibody
Application
Recommended Usage
Knockout Validated
HO‑1/HMOX1/HSP32
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
HO‑1/HMOX1/HSP32 knockout HeLa cell line.
Simple Western
25 µg/mL
Sample: A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line
Sample: A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line
Western Blot
0.5 µg/mL
Sample: A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line
Sample: A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF3776 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-1/HMOX1/HSP32
Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.
Long Name
Heme Oxygenase 1
Alternate Names
HMOX1, HO1, HSP32
Gene Symbol
HMOX1
UniProt
Additional HO-1/HMOX1/HSP32 Products
Product Documents for Human/Mouse HO-1/HMOX1/HSP32 Antibody
Product Specific Notices for Human/Mouse HO-1/HMOX1/HSP32 Antibody
For research use only
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